Hotspot

rps15-ycf1

SSC · 126861-127221 · 361 bp

Back to Diospyros

Pi 0.0241

MarkerSeek score 68.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 361
variable_sites: 39
pi: 0.0241
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	ACTTGAAATTCAACAGACCCTCT	TGGCAGTCGTTTCAGTATGT	39.1	45.0	57.9	57.2	476-512 bp	1.0	59.4
rps15-ycf1_p2	ACTTGAAATTCAACAGACCCTCT	TGGCAGTCGTTTCAGTATGTT	39.1	42.9	57.9	57.9	476-512 bp	1.0	57.1
rps15-ycf1_p3	CAGACCCTCTGTTTTCTTCT	TGGCAGTCGTTTCAGTATGT	45.0	45.0	54.6	57.2	463-499 bp	1.0	56.8
rps15-ycf1_p4	ACTTGAAATTCAACAGACCCTC	TGGCAGTCGTTTCAGTATGT	40.9	45.0	56.5	57.2	476-512 bp	1.0	56.1
rps15-ycf1_p5	ACTTGAAATTCAACAGACCCTCT	ATGGCAGTCGTTTCAGTATGT	39.1	42.9	57.9	57.7	477-513 bp	1.0	55.3