Hotspot

ccsA-ndhD

SSC · 118721-118958 · 238 bp

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Pi 0.0539

MarkerSeek score 73.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 238
variable_sites: 36
pi: 0.0539
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTGGGTTATGCGGCTCTTTT	CCGCGAGAGTTGTTTGTTTC	45.0	50.0	58.1	58.0	892-916 bp	1.0	86.4
ccsA-ndhD_p2	TTGGACCGTATTCGCGATTT	CCGCGAGAGTTGTTTGTTTC	45.0	50.0	58.0	58.0	533-557 bp	0.941176	86.4
ccsA-ndhD_p3	TTACTTGGACCGTATTCGCG	CCGCGAGAGTTGTTTGTTTC	50.0	50.0	58.1	58.0	537-561 bp	0.941176	85.7
ccsA-ndhD_p4	TTGGACCGTATTCGCGATTT	TTCTCTTGCCTTACCGGGTA	45.0	50.0	58.0	58.1	756-780 bp	0.941176	84.3
ccsA-ndhD_p5	TTGGACCGTATTCGCGATTT	TTTTTGATTCTGGACCGCGA	45.0	45.0	58.0	58.1	547-571 bp	0.882353	82.1