Hotspot

ndhF-rpl32

SSC · 115607-116560 · 954 bp

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Pi 0.0205
MarkerSeek score 73.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
954
variable_sites
103
pi
0.0205
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGGTATGATCCACGCATATTGA AGAGCTTTTAAGGATGCCCA 40.9 45.0 57.1 56.8 1046-1119 bp 0.882353 53.5
ndhF-rpl32_p2 AGGTATGATCCACGCATATTGA AAGAGCTTTTAAGGATGCCCA 40.9 42.9 57.1 57.5 1047-1120 bp 0.882353 51.5
ndhF-rpl32_p3 AGGTATGATCCACGCATATTGA AGAGCTTTTAAGGATGCCCAA 40.9 42.9 57.1 57.5 1046-1119 bp 0.882353 51.5
ndhF-rpl32_p4 AGGTATGATCCACGCATATTGAG AGAGCTTTTAAGGATGCCCA 43.5 45.0 58.2 56.8 1046-1119 bp 0.882353 51.2
ndhF-rpl32_p5 GGTATGATCCACGCATATTGAGA AGAGCTTTTAAGGATGCCCA 43.5 45.0 58.2 56.8 1045-1118 bp 0.882353 51.2