Hotspot

petA-psbJ

LSC · 66045-66793 · 749 bp

Back to Diospyros

Pi 0.0242

MarkerSeek score 72.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 749
variable_sites: 85
pi: 0.0242
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAAGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	38.1	50.0	55.5	57.6	803-950 bp	1.0	60.2
petA-psbJ_p2	TGAGAAAGTTCAATTGTCCGAA	ATTCCGGATTGGGTTCATCC	36.4	50.0	56.2	57.6	803-950 bp	1.0	58.3
petA-psbJ_p3	TTGAGAAAGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	36.4	50.0	56.2	57.6	804-951 bp	1.0	58.3
petA-psbJ_p4	TGAGAAAGTTCAATTGTCCGAAA	ATTCCGGATTGGGTTCATCC	34.8	50.0	56.9	57.6	803-950 bp	1.0	55.8
petA-psbJ_p5	TTGAGAAAGTTCAATTGTCCGAA	ATTCCGGATTGGGTTCATCC	34.8	50.0	56.9	57.6	804-951 bp	1.0	55.8