Hotspot

ndhC-trnV-UAC

LSC · 53093-54157 · 1065 bp

Back to Diospyros

Pi 0.0164

MarkerSeek score 72.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1065
variable_sites: 85
pi: 0.0164
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1166-1203 bp	1.0	87.2
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	1178-1215 bp	1.0	85.5
ndhC-trnV-UAC_p3	CTCCGGTCCTTTGCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	1185-1222 bp	1.0	84.8
ndhC-trnV-UAC_p4	GGGGCTAAAACTCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1165-1202 bp	1.0	84.4
ndhC-trnV-UAC_p5	TCCTTTGCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.5	57.8	1179-1216 bp	1.0	84.2