Hotspot

trnT-GGU-psbD

LSC · 33330-34852 · 1523 bp

Back to Diospyros

Pi 0.0143

MarkerSeek score 65.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1523
variable_sites: 103
pi: 0.0143
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1610-1663 bp	1.0	85.9
trnT-GGU-psbD_p2	GGCGTAAGTCATCGGTTCAA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1600-1653 bp	1.0	84.9
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	CAAAAACGAAACGGTCCCTC	50.0	50.0	58.3	57.6	1599-1652 bp	1.0	83.7
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	AGAGCAATAAACCGGACCAG	50.0	50.0	58.3	57.6	1623-1676 bp	1.0	83.6
trnT-GGU-psbD_p5	GGCGTAAGTCATCGGTTCAA	ATAAACCGGACCAGCCTACA	50.0	50.0	58.3	58.4	1617-1670 bp	1.0	83.4