Hotspot

trnC-GCA-petN

LSC · 29292-30048 · 757 bp

Back to Diospyros

Pi 0.0129

MarkerSeek score 66.60

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 757
variable_sites: 49
pi: 0.0129
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTGGATCCTTTTGGCGACA	AGCCCAAGCGAGACTTACTA	45.0	50.0	58.0	58.2	866-905 bp	1.0	87.2
trnC-GCA-petN_p2	TTGGATCCTTTTGGCGACAT	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.8	58.2	865-904 bp	1.0	85.6
trnC-GCA-petN_p3	TTTGGATCCTTTTGGCGACA	AGAGTCCACTTCTTCCCCAT	45.0	50.0	58.0	57.6	927-966 bp	1.0	85.4
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.4	58.2	829-868 bp	1.0	84.6
trnC-GCA-petN_p5	TTGGATCCTTTTGGCGACAT	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.8	57.6	926-965 bp	1.0	83.8