Hotspot

psbI-trnS-GCU

LSC · 9176-9327 · 152 bp

Back to Diospyros

Pi 0.0215

MarkerSeek score 67.80

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 152
variable_sites: 18
pi: 0.0215
unique_haplotype_count: 14
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CGTAATCCTGGACGTGAAGA	TAAAGCGGCGGATTGCTAAT	50.0	45.0	57.1	58.0	236-244 bp	1.0	82.5
psbI-trnS-GCU_p2	CGTAATCCTGGACGTGAAGA	ATTGGGAGAGATGGCTGAGT	50.0	50.0	57.1	58.1	260-268 bp	1.0	81.4
psbI-trnS-GCU_p3	CGTAATCCTGGACGTGAAGAA	TAAAGCGGCGGATTGCTAAT	47.6	45.0	57.8	58.0	236-244 bp	1.0	80.2
psbI-trnS-GCU_p4	CTCTCTTCATCTTCGGGTTCC	TAAAGCGGCGGATTGCTAAT	52.4	45.0	57.8	58.0	274-282 bp	1.0	80.0
psbI-trnS-GCU_p5	TCTCTTCATCTTCGGGTTCCT	TAAAGCGGCGGATTGCTAAT	47.6	45.0	58.2	58.0	273-281 bp	1.0	80.0