Hotspot

ycf1-rps15

SSC · 113508-113797 · 290 bp

Back to Dioscorea

Pi 0.0663

MarkerSeek score 73.40

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 290
variable_sites: 82
pi: 0.0663
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	TGTGTCTGGATGTGATGCTG	CAGCAGTCGTTGACGTTTTC	50.0	50.0	57.9	58.0	91-2279 bp	0.928571	88.3
ycf1-rps15_p2	TGTGTCTGGATGTGATGCTG	TTCTGGCGCATTTACCGTTA	50.0	45.0	57.9	57.9	91-2277 bp	0.928571	87.5
ycf1-rps15_p3	TGTGTCTGGATGTGATGCTG	TCCGCCATAAAAGGTCCAAG	50.0	50.0	57.9	57.9	91-2279 bp	0.928571	87.3
ycf1-rps15_p4	TGTGTCTGGATGTGATGCTG	CGCTCTTTGGGGTACTTGAA	50.0	50.0	57.9	57.8	91-2279 bp	0.928571	86.9
ycf1-rps15_p5	TGTGTCTGGATGTGATGCTG	AACACCATGCATTGATGGGT	50.0	45.0	57.9	58.1	90-2278 bp	0.904762	86.9