Hotspot

trnP-UGG-psaJ

LSC · 65382-65758 · 377 bp

Back to Dioscorea

Pi 0.0482

MarkerSeek score 72.70

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 377
variable_sites: 82
pi: 0.0482
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	CGAACTGAACTAAGAGCGCT	GCTTTCGAACCAGCATCCTA	50.0	50.0	58.0	58.0	91-2276 bp	0.97619	90.7
trnP-UGG-psaJ_p2	CGAACTGAACTAAGAGCGCT	CTGACATCCTTACCCTTGGC	50.0	55.0	58.0	58.0	91-2278 bp	0.97619	90.6
trnP-UGG-psaJ_p3	TATTTCTCCGGAATTGCGGG	GCTTTCGAACCAGCATCCTA	50.0	50.0	58.0	58.0	92-2277 bp	0.619048	74.7
trnP-UGG-psaJ_p4	CGAACTGAACTAAGAGCGCT	GCGAAATTTGAGTCACGTGG	50.0	50.0	58.0	58.0	91-3000 bp	0.595238	73.5
trnP-UGG-psaJ_p5	CCGGAATTGCGGGTATTGTA	GCTTTCGAACCAGCATCCTA	50.0	50.0	58.0	58.0	91-2276 bp	0.595238	72.9