Hotspot

petA-psbJ

LSC · 62242-63132 · 891 bp

Back to Dioscorea

Pi 0.0404

MarkerSeek score 75.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 891
variable_sites: 161
pi: 0.0404
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	GGGTTGGGTTCATCTCTGTAG	50.0	52.4	58.0	57.7	92-2279 bp	1.0	81.9
petA-psbJ_p2	GGCATCTGTGATTTTGGCAC	GGGTTGGGTTCATCTCTGTA	50.0	50.0	58.0	56.5	91-2276 bp	1.0	80.3
petA-psbJ_p3	TGGCATCTGTGATTTTGGCA	GGGTTGGGTTCATCTCTGTAG	45.0	52.4	58.4	57.7	92-2280 bp	1.0	78.9
petA-psbJ_p4	TGGCATCTGTGATTTTGGCA	GGGTTGGGTTCATCTCTGTA	45.0	50.0	58.4	56.5	91-2277 bp	1.0	77.3
petA-psbJ_p5	GCATCTGTGATTTTGGCACA	GGGTTGGGTTCATCTCTGTAG	45.0	52.4	57.3	57.7	92-2279 bp	1.0	76.0