Hotspot

psbM-trnD-GUC

LSC · 29227-30140 · 914 bp

Back to Dioscorea

Pi 0.0286

MarkerSeek score 74.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 914
variable_sites: 138
pi: 0.0286
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	ATTGATTCTTTCGACGGCCA	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.9	57.9	1496-2306 bp	0.642857	71.7
psbM-trnD-GUC_p2	ATTGATTCTTTCGACGGCCA	TCTCTTTCAAGGAGGCAACG	45.0	50.0	57.9	57.8	2054-2858 bp	0.642857	71.4
psbM-trnD-GUC_p3	TTGATTCTTTCGACGGCCAT	AGTTCAATCGGTCAGAGCAC	45.0	50.0	57.9	57.9	1495-2305 bp	0.571429	68.8
psbM-trnD-GUC_p4	TTGATTCTTTCGACGGCCAT	TCTCTTTCAAGGAGGCAACG	45.0	50.0	57.9	57.8	2053-2857 bp	0.571429	68.4
psbM-trnD-GUC_p5	TTGATTCTTTCGACGGCCAT	ATAGACCTCACCGGGATTGT	45.0	50.0	57.9	57.8	1515-2325 bp	0.571429	68.1