Hotspot

trnC-GCA-petN

LSC · 27282-28227 · 946 bp

Back to Dioscorea

Pi 0.0412
MarkerSeek score 80.50
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
946
variable_sites
207
pi
0.0412
unique_haplotype_count
35
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 CAAATCTGGGTGTCGCCTAA TACCCCTAGAGTCCACTCCT 50.0 55.0 57.9 58.0 652-2278 bp 0.97619 90.2
trnC-GCA-petN_p2 GGGTGTCGCCTAATCAACAA TACCCCTAGAGTCCACTCCT 50.0 55.0 58.2 58.0 645-2277 bp 0.97619 89.6
trnC-GCA-petN_p3 CAAATCTGGGTGTCGCCTAA AGTCCACTCCTTCCCCATAC 50.0 55.0 57.9 58.1 653-2279 bp 0.97619 89.2
trnC-GCA-petN_p4 CAAATCTGGGTGTCGCCTAA GTCCACTCCTTCCCCATACT 50.0 55.0 57.9 58.1 652-2278 bp 0.97619 89.2
trnC-GCA-petN_p5 CAAATCTGGGTGTCGCCTAA AGCCCAAGCGAGACTTACTA 50.0 50.0 57.9 58.2 654-2277 bp 0.97619 89.0