Hotspot

trnS-GCU-trnG-UCC

LSC · 7012-8027 · 1016 bp

Back to Dioscorea

Pi 0.0618
MarkerSeek score 79.30
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1016
variable_sites
269
pi
0.0618
unique_haplotype_count
35
primer_available
yes
alignment_reliability
0.95
markerseek_score
79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CCGTCGCTTTAATCCACTCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 358-2278 bp 1.0 82.1
trnS-GCU-trnG-UCC_p2 CCGTCGCTTTAATCCACTCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 357-2278 bp 1.0 82.0
trnS-GCU-trnG-UCC_p3 TCGCTTTAATCCACTCAGCC ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 355-2279 bp 1.0 81.9
trnS-GCU-trnG-UCC_p4 TCGCTTTAATCCACTCAGCC ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 354-2279 bp 1.0 81.9
trnS-GCU-trnG-UCC_p5 CAGATTAGCAATCCGTCGCT ACGAATCGCACTTTTACCACT 50.0 42.9 58.1 58.2 370-2279 bp 1.0 81.0