Hotspot

rpl23

LSC · 86707-86988 · 282 bp

Back to Diocirea

Pi 0.00236

MarkerSeek score 32.60

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: LSC
length_bp: 282
variable_sites: 1
pi: 0.00236
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	TGCTCGGGGTAGAAGTTTTG	TGAGACTAGAACTCATAGGGAAGA	50.0	41.7	57.8	57.7	383 bp	1.0	44.1
rpl23_p2	GCTCGGGGTAGAAGTTTTGT	TGAGACTAGAACTCATAGGGAAGA	50.0	41.7	57.8	57.7	382 bp	1.0	44.1
rpl23_p3	GTGCTCGGGGTAGAAGTTTT	TGAGACTAGAACTCATAGGGAAGA	50.0	41.7	57.8	57.7	384 bp	1.0	44.1
rpl23_p4	TGCTCGGGGTAGAAGTTTTG	TGAGACTAGAACTCATAGGGAAG	50.0	43.5	57.8	56.3	383 bp	1.0	41.2
rpl23_p5	GCTCGGGGTAGAAGTTTTGT	TGAGACTAGAACTCATAGGGAAG	50.0	43.5	57.8	56.3	382 bp	1.0	41.2