Hotspot

ndhF

SSC · 110792-113023 · 2232 bp

Back to Diocirea

Pi 0.00239

MarkerSeek score 53.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 8
pi: 0.00239
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GCTATAAAAATAGCCGCCCT	GAGGTAAGATCCGGTGAATCG	45.0	52.4	55.7	58.0	2405 bp	1.0	53.4
ndhF_p2	GCTATAAAAATAGCCGCCCT	AGGTAAGATCCGGTGAATCG	45.0	50.0	55.7	56.8	2404 bp	1.0	52.1
ndhF_p3	GCTATAAAAATAGCCGCCCTT	GAGGTAAGATCCGGTGAATCG	42.9	52.4	56.4	58.0	2405 bp	1.0	51.7
ndhF_p4	GCTATAAAAATAGCCGCCCTT	AGGTAAGATCCGGTGAATCG	42.9	50.0	56.4	56.8	2404 bp	1.0	50.3
ndhF_p5	GCTATAAAAATAGCCGCCCTTT	GAGGTAAGATCCGGTGAATCG	40.9	52.4	57.2	58.0	2405 bp	1.0	49.4