Hotspot

psaC-ndhE

SSC · 117891-118146 · 256 bp

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Pi 0.00521

MarkerSeek score 45.90

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 256
variable_sites: 2
pi: 0.00521
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACGCTCGAACACATTGAGTA	GCTATTGGACCGGCTATTGT	45.0	50.0	57.3	57.7	401 bp	1.0	72.6
psaC-ndhE_p2	CACGCTCGAACACATTGAGT	GCTATTGGACCGGCTATTGT	50.0	50.0	58.9	57.7	402 bp	1.0	71.5
psaC-ndhE_p3	ACGCTCGAACACATTGAGTA	AGCTATTGGACCGGCTATTG	45.0	50.0	57.3	57.4	402 bp	1.0	70.3
psaC-ndhE_p4	ACGCTCGAACACATTGAGTA	CAGCTATTGGACCGGCTATT	45.0	50.0	57.3	57.4	403 bp	1.0	70.2
psaC-ndhE_p5	ACGCTCGAACACATTGAGTA	TTGGACCGGCTATTGTTTCA	45.0	45.0	57.3	57.4	397 bp	1.0	70.1