Hotspot

psbN-psbH

LSC · 75513-75617 · 105 bp

Back to Diocirea

Pi 0.0127

MarkerSeek score 60.60

Primer available yes

Label psbN-psbH

Feature Metadata

label_name: psbN-psbH
feature_type: igs
region: LSC
length_bp: 105
variable_sites: 2
pi: 0.0127
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbN-psbH_p1	TAGTTGTTGGGAAGGTTGCC	GGCCCGATCTAGAACTGTTC	50.0	55.0	58.0	57.8	247 bp	1.0	78.8
psbN-psbH_p2	GCGGTATATAAGGCGTACCC	GGCCCGATCTAGAACTGTTC	55.0	55.0	57.7	57.8	222 bp	1.0	76.6
psbN-psbH_p3	GGCGTACCCGGTAAAACTTA	GGCCCGATCTAGAACTGTTC	50.0	55.0	57.6	57.8	211 bp	1.0	75.9
psbN-psbH_p4	TAAGGCGTACCCGGTAAAAC	GGCCCGATCTAGAACTGTTC	50.0	55.0	57.6	57.8	214 bp	1.0	75.9
psbN-psbH_p5	AAAGATGGCGACTAGGGTTG	GGCCCGATCTAGAACTGTTC	50.0	55.0	57.6	57.8	175 bp	1.0	75.9