Hotspot

rpl20-rps12

LSC · 70060-70855 · 796 bp

Back to Diocirea

Pi 0.00251

MarkerSeek score 60.40

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 796
variable_sites: 3
pi: 0.00251
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TCCGAGCTATATATCCGCGT	AAATGTCACGAAATCCCCCG	50.0	50.0	58.0	58.5	897-902 bp	1.0	75.7
rpl20-rps12_p2	CTTGAATGAGCCCCTCGAAA	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	952-957 bp	1.0	75.0
rpl20-rps12_p3	GAATGAGCCCCTCGAAAACT	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	949-954 bp	1.0	75.0
rpl20-rps12_p4	GAGCCCCTCGAAAACTTGAT	AAATGTCACGAAATCCCCCG	50.0	50.0	57.9	58.5	945-950 bp	1.0	75.0
rpl20-rps12_p5	AGCCCCTCGAAAACTTGATG	AAATGTCACGAAATCCCCCG	50.0	50.0	58.2	58.5	944-949 bp	1.0	74.5