Hotspot

ycf4-cemA

LSC · 61492-62210 · 719 bp

Back to Diocirea

Pi 0.000927

MarkerSeek score 59.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 1
pi: 0.000927
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TATTCCGTTGACTCGCACTG	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	894-895 bp	1.0	79.5
ycf4-cemA_p2	CTTGCGCGTACCAATTGAAG	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	819-820 bp	1.0	78.6
ycf4-cemA_p3	GAACAAAAAGCCGCGGAATT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.2	58.1	848-849 bp	1.0	77.8
ycf4-cemA_p4	CTATTCCGTTGACTCGCACT	GAGAAATCCACCAGGGCAAA	50.0	50.0	57.7	58.1	895-896 bp	1.0	77.3
ycf4-cemA_p5	TTTCTTGCGCGTACCAATTG	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.7	58.1	822-823 bp	1.0	77.0