Hotspot

atpB-rbcL

LSC · 55734-56517 · 784 bp

Back to Diocirea

Pi 0.0034

MarkerSeek score 60.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 784
variable_sites: 4
pi: 0.0034
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGAAACCCCAGAACCAGAAG	AACACCCGCTTTGAATCCAA	55.0	45.0	58.1	58.3	874-879 bp	1.0	77.2
atpB-rbcL_p2	ATGATTTGGACGATACGCCC	AACACCCGCTTTGAATCCAA	50.0	45.0	58.1	58.3	915-920 bp	1.0	77.0
atpB-rbcL_p3	CGGCGGAAAAGCTACATCTA	AACACCCGCTTTGAATCCAA	50.0	45.0	57.8	58.3	946-951 bp	1.0	76.2
atpB-rbcL_p4	GGACCGATGATTTGGACGAT	AACACCCGCTTTGAATCCAA	50.0	45.0	57.8	58.3	921-926 bp	1.0	76.0
atpB-rbcL_p5	GGAAACCCCAGAACCAGAAG	TAACACCCGCTTTGAATCCA	55.0	45.0	58.1	57.4	875-880 bp	1.0	75.1