Hotspot

petN-psbM

LSC · 29584-30567 · 984 bp

Back to Diocirea

Pi 0.00407

MarkerSeek score 61.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 984
variable_sites: 6
pi: 0.00407
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ACTCGTAGTGTGGGGAAGAA	TGCTACTGCGCTATTCATTCT	50.0	42.9	58.0	57.5	1090-1094 bp	1.0	69.0
petN-psbM_p2	TCGTAGTGTGGGGAAGAAGT	TGCTACTGCGCTATTCATTCT	50.0	42.9	58.0	57.5	1088-1092 bp	1.0	69.0
petN-psbM_p3	CGTAGTGTGGGGAAGAAGTG	TGCTACTGCGCTATTCATTCT	55.0	42.9	57.9	57.5	1087-1091 bp	1.0	68.4
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCGCTATTCATTCT	50.0	42.9	58.2	57.5	1142-1146 bp	1.0	67.8
petN-psbM_p5	GTAGTGTGGGGAAGAAGTGG	TGCTACTGCGCTATTCATTCT	55.0	42.9	57.5	57.5	1086-1090 bp	1.0	65.3