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psbK-psbI

LSC · 8122-8495 · 374 bp

Back to Diocirea

Pi 0.00357

MarkerSeek score 45.10

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 2
pi: 0.00357
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTTGTTTGGCAAGCTGCT	ACGCCCCGGATCATTAGATA	45.0	50.0	58.1	58.1	499 bp	1.0	79.3
psbK-psbI_p2	CTTTTGTTTGGCAAGCTGCT	TACGCCCCGGATCATTAGAT	45.0	50.0	58.1	58.1	500 bp	1.0	79.3
psbK-psbI_p3	GTTTGGCAAGCTGCTGTAAG	ACGCCCCGGATCATTAGATA	50.0	50.0	57.9	58.1	494 bp	1.0	79.3
psbK-psbI_p4	GTTTGGCAAGCTGCTGTAAG	TACGCCCCGGATCATTAGAT	50.0	50.0	57.9	58.1	495 bp	1.0	79.3
psbK-psbI_p5	CTTTTGTTTGGCAAGCTGCT	TTACGCCCCGGATCATTAGA	45.0	50.0	58.1	58.3	501 bp	1.0	77.5