Hotspot

rps16-trnQ-UUG

LSC · 6381-7528 · 1148 bp

Back to Diocirea

Pi 0.00348

MarkerSeek score 61.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1148
variable_sites: 6
pi: 0.00348
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AACGGATCGTGTCCTTCAAG	GAAATTGAAATGGGGCGTGG	50.0	50.0	57.9	58.0	1296 bp	1.0	79.7
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1279 bp	1.0	79.2
rps16-trnQ-UUG_p3	AACGGATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.9	58.1	1238 bp	1.0	79.1
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1221 bp	1.0	78.7
rps16-trnQ-UUG_p5	CAACGGATCGTGTCCTTCAA	GAAATTGAAATGGGGCGTGG	50.0	50.0	58.2	58.0	1297 bp	1.0	78.6