Hotspot

trnK-UUU-rps16

LSC · 4291-5281 · 991 bp

Back to Diocirea

Pi 0.00437
MarkerSeek score 61.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
991
variable_sites
6
pi
0.00437
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 1084-1103 bp 1.0 66.0
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 1083-1102 bp 1.0 66.0
trnK-UUU-rps16_p3 CAGTCGCGGTCTTACAAACT GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 1168-1187 bp 1.0 65.5
trnK-UUU-rps16_p4 TCAGTCGCGGTCTTACAAAC GTGCTCAACCCACAGGAAC 50.0 57.9 58.2 58.7 1169-1188 bp 1.0 65.5
trnK-UUU-rps16_p5 TCTAGCCGCACTTAAAAGCC GTGCTCAACCCACAGGAAC 50.0 57.9 58.3 58.7 1098-1117 bp 1.0 65.2