Hotspot

rpl16

LSC · 77972-79469 · 1498 bp

Back to Dinebra

Pi 0.00838

MarkerSeek score 51.10

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1498
variable_sites: 18
pi: 0.00838
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCTCAATTCCTCGGCAATCG	TCAAAATTGCAGGCCGTCTA	50.0	45.0	58.1	57.8	2223-2264 bp	1.0	80.6
rpl16_p2	ATCGCGCCAAAAACTCTAGT	TCAAAATTGCAGGCCGTCTA	45.0	45.0	57.9	57.8	2207-2248 bp	1.0	80.3
rpl16_p3	TTTCTAGAGGCATTTGGGGC	TCAAAATTGCAGGCCGTCTA	50.0	45.0	57.9	57.8	2062-2103 bp	1.0	80.1
rpl16_p4	TCGCGCCAAAAACTCTAGTT	TCAAAATTGCAGGCCGTCTA	45.0	45.0	58.1	57.8	2206-2247 bp	1.0	80.0
rpl16_p5	TCAGCAATAGTGTCCTTGCC	TCAAAATTGCAGGCCGTCTA	50.0	45.0	57.9	57.8	2433-2474 bp	1.0	79.9