Hotspot

ccsA

SSC · 105928-106896 · 969 bp

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Pi 0.00619

MarkerSeek score 47.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 9
pi: 0.00619
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGACACGCTGCTCTTAGGA	TCTGGACCACGAGAACTCTT	50.0	50.0	57.9	58.0	1435 bp	1.0	80.1
ccsA_p2	TAGACACGCTGCTCTTAGGA	TTCTGGACCACGAGAACTCT	50.0	50.0	57.9	58.0	1436 bp	1.0	80.1
ccsA_p3	CATTTTGGAGCGGCAATGAG	TCTGGACCACGAGAACTCTT	50.0	50.0	58.1	58.0	1939 bp	0.333333	52.6
ccsA_p4	CATTTTGGAGCGGCAATGAG	TTCTGGACCACGAGAACTCT	50.0	50.0	58.1	58.0	1940 bp	0.333333	52.6
ccsA_p5	CATTTTGGAGCGGCAATGAG	GGATTCTGGACCACGAGAAC	50.0	55.0	58.1	58.0	1943 bp	0.333333	52.6