Hotspot

rpl14-rpl16

LSC · 77852-77971 · 120 bp

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Pi 0.0192

MarkerSeek score 66.10

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 120
variable_sites: 3
pi: 0.0192
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	GCTCCACTGTTATCCGCTAC	AATGCCCATACGAAGCCAAT	55.0	45.0	58.1	57.9	191-207 bp	1.0	83.5
rpl14-rpl16_p2	GCTCCACTGTTATCCGCTAC	ATGCCCATACGAAGCCAATT	55.0	45.0	58.1	57.9	190-206 bp	1.0	83.5
rpl14-rpl16_p3	AGCTCCACTGTTATCCGCTA	AATGCCCATACGAAGCCAAT	50.0	45.0	58.2	57.9	192-208 bp	1.0	82.9
rpl14-rpl16_p4	AGCTCCACTGTTATCCGCTA	ATGCCCATACGAAGCCAATT	50.0	45.0	58.2	57.9	191-207 bp	1.0	82.9
rpl14-rpl16_p5	GCTCCACTGTTATCCGCTAC	TGCCCATACGAAGCCAATTT	55.0	45.0	58.1	58.2	189-205 bp	1.0	82.8