Hotspot

rbcL-psaI

LSC · 56677-57561 · 885 bp

Back to Dinebra

Pi 0.0124

MarkerSeek score 54.40

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 16
pi: 0.0124
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	AAGCCGGTAGATACCATCGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	978-979 bp	1.0	81.5
rbcL-psaI_p2	CAAGCCGGTAGATACCATCG	CCATTGCAATTGCCGGAAAT	55.0	45.0	57.9	58.0	979-980 bp	1.0	81.1
rbcL-psaI_p3	AAGCCGGTAGATACCATCGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	984-985 bp	1.0	80.9
rbcL-psaI_p4	CAAGCCGGTAGATACCATCG	AAGAAGCCATTGCAATTGCC	55.0	45.0	57.9	57.9	985-986 bp	1.0	80.4
rbcL-psaI_p5	AGCCGGTAGATACCATCGAT	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.8	58.0	977-978 bp	1.0	79.8