Hotspot

ndhC-trnV-UAC

LSC · 50568-51468 · 901 bp

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Pi 0.00819

MarkerSeek score 64.60

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 901
variable_sites: 11
pi: 0.00819
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGTCCTTCATTAATCGGGGC	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.4	57.9	1037-1060 bp	1.0	77.5
ndhC-trnV-UAC_p2	GGTCCTTCATTAATCGGGGC	AGCCCAATGCAGAAAAATGC	55.0	45.0	58.4	57.9	1130-1153 bp	1.0	77.2
ndhC-trnV-UAC_p3	GGTCCTTCATTAATCGGGGC	CGGTTCGAATCCGTATAGCC	55.0	55.0	58.4	58.3	1038-1061 bp	1.0	75.7
ndhC-trnV-UAC_p4	GTCCTTCATTAATCGGGGCT	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.4	57.9	1036-1059 bp	1.0	75.6
ndhC-trnV-UAC_p5	GTCCTTCATTAATCGGGGCT	AGCCCAATGCAGAAAAATGC	50.0	45.0	57.4	57.9	1129-1152 bp	1.0	75.2