Hotspot

psaA-pafI

LSC · 41951-42599 · 649 bp

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Pi 0.0105

MarkerSeek score 67.30

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 10
pi: 0.0105
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	GGGGCCCTTAGCTATTGTTC	TATAGCGCTTACTCCGGGAA	55.0	50.0	58.0	58.0	837-845 bp	1.0	81.9
psaA-pafI_p2	GGGGCCCTTAGCTATTGTTC	GCGCTTACTCCGGGAAATTA	55.0	50.0	58.0	58.1	833-841 bp	1.0	81.6
psaA-pafI_p3	GGGGCCCTTAGCTATTGTTC	TGGTTTGATCAAGCTGCTGA	55.0	45.0	58.0	57.7	872-880 bp	1.0	79.8
psaA-pafI_p4	GGGGCCCTTAGCTATTGTTC	GGTTGAAGATTACGAAGCGC	55.0	50.0	58.0	57.6	796-804 bp	1.0	78.7
psaA-pafI_p5	TGTTCTTGAGAAATGCCCGG	TATAGCGCTTACTCCGGGAA	50.0	50.0	58.5	58.0	822-830 bp	1.0	78.4