Hotspot

atpI-atpH

LSC · 31906-32716 · 811 bp

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Pi 0.00421

MarkerSeek score 65.90

Primer available yes

Label atpI-atpH

Feature Metadata

label_name: atpI-atpH
feature_type: igs
region: LSC
length_bp: 811
variable_sites: 5
pi: 0.00421
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-atpH_p1	CCGGTCATGTTTCTCGGATT	CTCTACAGCTTGTCCTGCAG	50.0	55.0	58.0	58.0	993-1024 bp	1.0	83.0
atpI-atpH_p2	CCGGTCATGTTTCTCGGATT	AGCCAATCCAGCAGCAATAA	50.0	45.0	58.0	57.9	930-961 bp	1.0	82.1
atpI-atpH_p3	CCGGTCATGTTTCTCGGATT	AAGCAGCAGCAATTAGTGGA	50.0	45.0	58.0	57.8	907-938 bp	1.0	81.7
atpI-atpH_p4	CCGGTCATGTTTCTCGGATT	GCAGTACCTTGACCAACTCC	50.0	55.0	58.0	58.2	974-1005 bp	1.0	81.6
atpI-atpH_p5	CCGGTCATGTTTCTCGGATT	GCCAATCCAGCAGCAATAAC	50.0	50.0	58.0	57.8	929-960 bp	1.0	81.6