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rpoC2-rps2

LSC · 29893-30194 · 302 bp

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Pi 0.0177

MarkerSeek score 50.20

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 302
variable_sites: 8
pi: 0.0177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	ATGAGACATCAGAACCCCCA	ACCAAAATGAACTCCCGCTT	50.0	45.0	58.0	58.0	409-416 bp	1.0	81.9
rpoC2-rps2_p2	TGAGACATCAGAACCCCCAT	ACCAAAATGAACTCCCGCTT	50.0	45.0	58.0	58.0	408-415 bp	1.0	81.9
rpoC2-rps2_p3	ATGAGACATCAGAACCCCCA	TTTTCCCTGACTTGCTGCAT	50.0	45.0	58.0	58.0	553-560 bp	1.0	81.5
rpoC2-rps2_p4	TGAGACATCAGAACCCCCAT	TTTTCCCTGACTTGCTGCAT	50.0	45.0	58.0	58.0	552-559 bp	1.0	81.5
rpoC2-rps2_p5	TGAGACATCAGAACCCCCAT	ACGAGACCTTATTGCAGCTG	50.0	50.0	58.0	58.0	621-628 bp	1.0	81.2