Hotspot

trnS-CGA-trnT-GGU

LSC · 13555-14550 · 996 bp

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Pi 0.0161

MarkerSeek score 57.90

Primer available yes

Label trnS-CGA-trnT-GGU

Feature Metadata

label_name: trnS-CGA-trnT-GGU
feature_type: igs
region: LSC
length_bp: 996
variable_sites: 24
pi: 0.0161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-CGA-trnT-GGU_p1	ACGAATCACACTTTTACCACT	GGTCTTTCCGTGGCATTACT	38.1	50.0	55.5	57.9	1139-1146 bp	1.0	52.9
trnS-CGA-trnT-GGU_p2	ACGAATCACACTTTTACCACT	TCCGTGGCATTACTCTACCA	38.1	50.0	55.5	58.1	1133-1140 bp	1.0	52.6
trnS-CGA-trnT-GGU_p3	AGAACGAATCACACTTTTACCAC	GGTCTTTCCGTGGCATTACT	39.1	50.0	57.3	57.9	1142-1149 bp	1.0	51.8
trnS-CGA-trnT-GGU_p4	GAACGAATCACACTTTTACCACT	GGTCTTTCCGTGGCATTACT	39.1	50.0	57.3	57.9	1141-1148 bp	1.0	51.8
trnS-CGA-trnT-GGU_p5	GAACGAATCACACTTTTACCACT	TCCGTGGCATTACTCTACCA	39.1	50.0	57.3	58.1	1135-1142 bp	1.0	51.5