Hotspot

ycf1

SSC · 128063-133747 · 5685 bp

Back to Dimocarpus

Pi 0.01

MarkerSeek score 45.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5685
variable_sites: 57
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCCAAGTAGCGGTTTTACG	AGGGGGACTAAACAGGAACA	50.0	50.0	57.9	57.9	1346-1352 bp	1.0	79.5
ycf1_p2	AAACAGTTCCCAAGTAGCGG	AGGGGGACTAAACAGGAACA	50.0	50.0	58.1	57.9	1353-1359 bp	1.0	79.3
ycf1_p3	CAGTTCCCAAGTAGCGGTTT	AGGGGGACTAAACAGGAACA	50.0	50.0	58.1	57.9	1350-1356 bp	1.0	79.3
ycf1_p4	TCACTTGTTTTGCTGGTCCA	AGGGGGACTAAACAGGAACA	45.0	50.0	57.9	57.9	1255-1261 bp	1.0	79.2
ycf1_p5	TCCCAAGTAGCGGTTTTACG	CGAGGAAACGGAAGAGATCC	50.0	55.0	57.9	57.8	1257-1263 bp	1.0	78.9