Hotspot

ndhF-rpl32

SSC · 116236-117173 · 938 bp

Back to Dimocarpus

Pi 0.0702

MarkerSeek score 83.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 938
variable_sites: 63
pi: 0.0702
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCTGCCGTTGGAAAAAGTAC	GAGTGAAAGTGCGGGATAGG	50.0	55.0	58.0	58.1	1631-1641 bp	1.0	84.6
ndhF-rpl32_p2	GCTGCCGTTGGAAAAAGTAC	GGAGTGAAAGTGCGGGATAG	50.0	55.0	58.0	58.1	1632-1642 bp	1.0	84.6
ndhF-rpl32_p3	TTTGCTGCCGTTGGAAAAAG	GAGTGAAAGTGCGGGATAGG	45.0	55.0	58.1	58.1	1634-1644 bp	1.0	84.5
ndhF-rpl32_p4	TTTGCTGCCGTTGGAAAAAG	GGAGTGAAAGTGCGGGATAG	45.0	55.0	58.1	58.1	1635-1645 bp	1.0	84.5
ndhF-rpl32_p5	TTTTTGACAAGCACTTGCCG	GAGTGAAAGTGCGGGATAGG	45.0	55.0	58.1	58.1	2064-2074 bp	1.0	83.3