Hotspot

petD-rpoA

LSC · 80577-80828 · 252 bp

Back to Dimocarpus

Pi 0.0083

MarkerSeek score 61.50

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 2
pi: 0.0083
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	CAGTAGCGACAACCGTCTTT	GAGCCAAGACGACCTTATGA	50.0	50.0	58.2	57.0	427-438 bp	1.0	72.9
petD-rpoA_p2	CAGTAGCGACAACCGTCTTT	AGAGCCAAGACGACCTTATG	50.0	50.0	58.2	57.0	428-439 bp	1.0	72.9
petD-rpoA_p3	AGCGACAACCGTCTTTTTGA	GAGCCAAGACGACCTTATGA	45.0	50.0	58.3	57.0	423-434 bp	1.0	72.0
petD-rpoA_p4	AGCGACAACCGTCTTTTTGA	AGAGCCAAGACGACCTTATG	45.0	50.0	58.3	57.0	424-435 bp	1.0	71.9
petD-rpoA_p5	CAGTAGCGACAACCGTCTTT	GAGCCAAGACGACCTTATGAA	50.0	47.6	58.2	57.8	427-438 bp	1.0	70.7