Hotspot

psaJ-rpl33

LSC · 69753-70253 · 501 bp

Back to Dimocarpus

Pi 0.0222

MarkerSeek score 56.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 501
variable_sites: 11
pi: 0.0222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CTCTGGTTCGGGTCTTTAGC	TCGTTTCGAACACAACTGGT	55.0	45.0	58.0	58.0	648-653 bp	1.0	82.0
psaJ-rpl33_p2	CTCTGGTTCGGGTCTTTAGC	GCAAACGCCTACGAAAAGAC	55.0	50.0	58.0	58.1	1047-1055 bp	1.0	81.8
psaJ-rpl33_p3	ACATATCTTTCCGTGGCACC	TCGTTTCGAACACAACTGGT	50.0	45.0	58.0	58.0	681-686 bp	1.0	81.7
psaJ-rpl33_p4	CTCTGGTTCGGGTCTTTAGC	AACGCCTACGAAAAGACCTC	55.0	50.0	58.0	57.9	1044-1052 bp	1.0	81.6
psaJ-rpl33_p5	ACATATCTTTCCGTGGCACC	GCAAACGCCTACGAAAAGAC	50.0	50.0	58.0	58.1	1080-1088 bp	1.0	81.5