Hotspot

psbZ-trnG-GCC

LSC · 38148-38698 · 551 bp

Back to Dimocarpus

Pi 0.0207

MarkerSeek score 58.40

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 551
variable_sites: 10
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	GGGTAGAGCAGTTTGGTAGC	55.0	55.0	58.0	58.0	877-960 bp	1.0	84.7
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCG	CTTGAGGTCACGGGTTCAAA	55.0	50.0	58.0	58.0	841-924 bp	1.0	84.7
psbZ-trnG-GCC_p3	CTTCTCCTGATGGTTGGTCG	GTAATAGTGCACCGACACGT	55.0	50.0	58.0	58.0	1192-1275 bp	1.0	84.5
psbZ-trnG-GCC_p4	CAAGACCGGAGCTATCAACC	GTAATAGTGCACCGACACGT	55.0	50.0	58.1	58.0	1661-1743 bp	1.0	84.2
psbZ-trnG-GCC_p5	ACGAAAAAGCCAGGCAAAAC	GTAATAGTGCACCGACACGT	45.0	50.0	58.1	58.0	1769-1851 bp	1.0	84.1