Hotspot

trnE-UUC-trnT-GGU

LSC · 32491-33276 · 786 bp

Back to Dimocarpus

Pi 0.0166

MarkerSeek score 61.70

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 13
pi: 0.0166
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CATAGTACCCTACCCCCAGG	GAACCGATGACTTACGCCTT	60.0	50.0	58.0	58.0	924-927 bp	1.0	81.0
trnE-UUC-trnT-GGU_p2	CATAGTACCCTACCCCCAGG	CCATGGCGTTACTCTACCAC	60.0	55.0	58.0	58.1	903-906 bp	1.0	80.6
trnE-UUC-trnT-GGU_p3	CATAGTACCCTACCCCCAGG	TGGCGTTACTCTACCACTGA	60.0	50.0	58.0	58.1	900-903 bp	1.0	80.4
trnE-UUC-trnT-GGU_p4	CATAGTACCCTACCCCCAGG	CCAAAAAGCCCCTTATCGGA	60.0	50.0	58.0	57.9	947-950 bp	1.0	80.0
trnE-UUC-trnT-GGU_p5	CATAGTACCCTACCCCCAGG	CCGATGACTTACGCCTTACC	60.0	55.0	58.0	58.2	921-924 bp	1.0	79.9