Hotspot

rps16-trnQ-UUG

LSC · 6223-7249 · 1027 bp

Back to Dimocarpus

Pi 0.0117
MarkerSeek score 64.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1027
variable_sites
12
pi
0.0117
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCGAAAACGGTGGATGGAAA GAAATTGAAATGGGGCGTGG 45.0 50.0 57.7 58.0 1201-1203 bp 1.0 79.0
rps16-trnQ-UUG_p2 TCGAAAACGGTGGATGGAAA GGAAATTGAAATGGGGCGTG 45.0 50.0 57.7 58.0 1202-1204 bp 1.0 79.0
rps16-trnQ-UUG_p3 ACACGTTGCTTTCTACCACA GAAATTGAAATGGGGCGTGG 45.0 50.0 57.7 58.0 1151-1153 bp 1.0 78.6
rps16-trnQ-UUG_p4 ACACGTTGCTTTCTACCACA GGAAATTGAAATGGGGCGTG 45.0 50.0 57.7 58.0 1152-1154 bp 1.0 78.6
rps16-trnQ-UUG_p5 TCGAAAACGGTGGATGGAAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.7 58.1 1143-1145 bp 1.0 78.5