Hotspot

trnN-GUU-trnR-ACG

IRa · 134345-134950 · 606 bp

Back to Dillenia

Pi 0.00864

MarkerSeek score 68.00

Primer available yes

Label trnN-GUU-trnR-ACG

Feature Metadata

label_name: trnN-GUU-trnR-ACG
feature_type: igs
region: IRa
length_bp: 606
variable_sites: 5
pi: 0.00864
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-trnR-ACG_p1	CCGTTGTTCCTCAGTAGCTC	CAGAGGATTAGAGCACGTGG	55.0	55.0	58.0	57.8	720-747 bp	1.0	81.7
trnN-GUU-trnR-ACG_p2	TGGGTTGAGAACGGGAATTG	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.1	57.8	761-788 bp	1.0	80.8
trnN-GUU-trnR-ACG_p3	TGGTCGTAGGTTCGAATCCT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.2	57.8	671-698 bp	1.0	80.8
trnN-GUU-trnR-ACG_p4	GGGTTGAGAACGGGAATTGA	CAGAGGATTAGAGCACGTGG	50.0	55.0	57.8	57.8	760-787 bp	1.0	80.0
trnN-GUU-trnR-ACG_p5	CGGTCGGCTGTTAACTGATT	CAGAGGATTAGAGCACGTGG	50.0	55.0	58.3	57.8	690-717 bp	1.0	79.7