Hotspot

psaJ-rpl33

LSC · 71051-71401 · 351 bp

Back to Dillenia

Pi 0.0399

MarkerSeek score 76.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 351
variable_sites: 14
pi: 0.0399
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TGGCACCGGTACTAAGTACT	GTTACTCGGGCATCTTTCCC	50.0	55.0	57.8	58.3	487-714 bp	1.0	77.4
psaJ-rpl33_p2	GTTCGGGTCTTTAGCAGGTC	GTTACTCGGGCATCTTTCCC	55.0	55.0	58.3	58.3	462-689 bp	1.0	77.1
psaJ-rpl33_p3	GTGGCACCGGTACTAAGTAC	GTTACTCGGGCATCTTTCCC	55.0	55.0	57.7	58.3	488-715 bp	1.0	76.9
psaJ-rpl33_p4	TGGCACCGGTACTAAGTACT	TACTCGGGCATCTTTCCCTT	50.0	50.0	57.8	58.4	485-712 bp	1.0	76.7
psaJ-rpl33_p5	TGGCACCGGTACTAAGTACT	TTACTCGGGCATCTTTCCCT	50.0	50.0	57.8	58.4	486-713 bp	1.0	76.7