Hotspot

petA-psbJ

LSC · 66629-67745 · 1117 bp

Back to Dillenia

Pi 0.00404

MarkerSeek score 64.10

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1117
variable_sites: 4
pi: 0.00404
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTATCCAAGGCCTTTTGT	TGGCATTCTTGTGATCGGTT	45.0	45.0	57.4	57.8	1176-1302 bp	1.0	78.0
petA-psbJ_p2	ACGTATCCAAGGCCTTTTGT	ACTGGCATTCTTGTGATCGG	45.0	50.0	57.4	58.3	1178-1304 bp	1.0	77.6
petA-psbJ_p3	ACGTATCCAAGGCCTTTTGT	CTGGCATTCTTGTGATCGGT	45.0	50.0	57.4	58.3	1177-1303 bp	1.0	77.6
petA-psbJ_p4	ACGTATCCAAGGCCTTTTGT	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.4	58.3	1136-1262 bp	1.0	77.1
petA-psbJ_p5	ACGTATCCAAGGCCTTTTGT	CATATTCCGGGTTGGGTTCA	45.0	50.0	57.4	57.6	1132-1258 bp	1.0	76.2