Hotspot

trnF-GAA-ndhJ

LSC · 51443-52144 · 702 bp

Back to Dillenia

Pi 0.0157

MarkerSeek score 67.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 11
pi: 0.0157
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	50.0	55.0	58.1	58.0	807-940 bp	1.0	80.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	808-941 bp	1.0	79.9
trnF-GAA-ndhJ_p3	ATGAGGATAACACACCGGGA	GTTGGATAGGATGGCCCTTG	50.0	55.0	58.1	58.0	870-1003 bp	1.0	79.8
trnF-GAA-ndhJ_p4	ATGAGGATAACACACCGGGA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	871-1004 bp	1.0	79.2
trnF-GAA-ndhJ_p5	TGAGGATAACACACCGGGAA	GTTGGATAGGATGGCCCTTG	50.0	55.0	58.4	58.0	869-1002 bp	1.0	78.0