Hotspot

rpoB-trnC-GCA

LSC · 27919-29029 · 1111 bp

Back to Dillenia

Pi 0.00281

MarkerSeek score 64.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1111
variable_sites: 3
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TCTTCTGCCAAGCCTTGATC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1274-1392 bp	1.0	81.1
rpoB-trnC-GCA_p2	TACCTCATTTCCATCCCCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1197-1315 bp	1.0	80.8
rpoB-trnC-GCA_p3	CTTCTGCCAAGCCTTGATCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1273-1391 bp	1.0	80.7
rpoB-trnC-GCA_p4	TGCCAAGCCTTGATCAATGA	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.8	58.1	1269-1387 bp	1.0	80.4
rpoB-trnC-GCA_p5	TTCTGCCAAGCCTTGATCAA	CCGGATTTGAACTGGGGAAA	45.0	50.0	57.7	58.1	1272-1390 bp	1.0	79.8