Hotspot

trnS-GCU-trnG-GCC

LSC · 8782-9828 · 1047 bp

Back to Dillenia

Pi 0.128
MarkerSeek score 94.00
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1047
variable_sites
105
pi
0.128
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.79
markerseek_score
94.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CCGACGCTTTAATCCACTCA ACGAATCGCACTTTTACCACT 50.0 42.9 58.0 58.2 935-1112 bp 1.0 82.1
trnS-GCU-trnG-GCC_p2 CCGACGCTTTAATCCACTCA ACGAATCGCACTTTTACCAC 50.0 45.0 58.0 56.8 935-1112 bp 1.0 82.0
trnS-GCU-trnG-GCC_p3 ACGGAAAGAGAGGGATTCGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.2 58.2 993-1170 bp 1.0 80.7
trnS-GCU-trnG-GCC_p4 ACGGAAAGAGAGGGATTCGA ACGAATCGCACTTTTACCAC 50.0 45.0 58.2 56.8 993-1170 bp 1.0 80.6
trnS-GCU-trnG-GCC_p5 ACGAAAACGGAAAGAGAGGG ACGAATCGCACTTTTACCACT 50.0 42.9 57.8 58.2 999-1176 bp 1.0 80.5