Hotspot

rps16-trnQ-UUG

LSC · 6443-7420 · 978 bp

Back to Dillenia

Pi 0.0043
MarkerSeek score 65.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
978
variable_sites
4
pi
0.0043
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
65.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TCCAATGGTGGGCGTAAAAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.0 58.1 1093-1658 bp 1.0 82.3
rps16-trnQ-UUG_p2 TTCCAATGGTGGGCGTAAAA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.0 58.1 1094-1659 bp 1.0 82.3
rps16-trnQ-UUG_p3 TCCAATGGTGGGCGTAAAAA AGAAATCTCAATGGGGCGTG 45.0 50.0 58.0 58.2 1152-1717 bp 1.0 80.9
rps16-trnQ-UUG_p4 TTCCAATGGTGGGCGTAAAA AGAAATCTCAATGGGGCGTG 45.0 50.0 58.0 58.2 1153-1718 bp 1.0 80.9
rps16-trnQ-UUG_p5 ATGGTGGGCGTAAAAATCCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.8 58.1 1089-1654 bp 1.0 80.6