Hotspot

trnK-UUU-rps16

LSC · 4494-5241 · 748 bp

Back to Dillenia

Pi 0.0179
MarkerSeek score 66.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
748
variable_sites
11
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC AAGGAGCTTCGCCTTAATGA 55.0 45.0 57.9 56.9 882-919 bp 1.0 75.7
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AGGAGCTTCGCCTTAATGAA 55.0 45.0 57.9 56.9 881-918 bp 1.0 75.7
trnK-UUU-rps16_p3 CCGATGGTATGGACGAATCC AGTTTTTAAGGAGCTTCGCCT 55.0 42.9 57.9 57.9 889-926 bp 1.0 75.2
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA AAGGAGCTTCGCCTTAATGA 50.0 45.0 58.3 56.9 830-867 bp 1.0 74.1
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA AGGAGCTTCGCCTTAATGAA 50.0 45.0 58.3 56.9 829-866 bp 1.0 74.1