Hotspot

trnH-GUG-psbA

LSC · 210-555 · 346 bp

Back to Dillenia

Pi 0.0301

MarkerSeek score 61.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 346
variable_sites: 8
pi: 0.0301
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	55.0	55.0	58.1	57.9	334-413 bp	1.0	84.2
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	348-427 bp	1.0	84.1
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	347-426 bp	1.0	84.1
trnH-GUG-psbA_p4	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	337-416 bp	1.0	84.1
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	349-428 bp	1.0	84.0